Biopharmaceutical harvest and clarification

Harvest and clarification solutions as advanced as your processes

Elevate the efficiency of your biopharmaceutical manufacturing process with next-generation technology.

Btec photoshoot 3M™ Harvest RC Centrate lab worker using wheel to elevate holder - 55

Overview Fiber chromatography Depth filtration Product comparison School of Purification Next Steps Ask an expert

Achieve your innovation goals with solutions from Solventum

Today’s increasingly complex and innovative therapeutics require equally innovative technologies. Higher cell densities, the need to scale up or down rapidly, and the goal of reaching market quickly with a high-quality product are key challenges for recombinant protein harvest and clarification. Downstream purification processes and the manufacturing facilities’ flexibility and efficiency depend on your choice of harvest and clarification products.

Push the boundaries of your process by choosing our innovative anion exchange (AEX) fiber chromatography. AEX fibrous functional media separates by charge, effectively capturing contaminants and impurities that are negatively charged at the harvest cell culture pH, including cells, cell debris, host cell proteins (HCP) and DNA. The positively charged molecule of interest can flow through.

Infographic of various molecules separated by charge

Fiber chromatographic solutions for efficiency and yield

Advances in biopharmaceutical development mean rising cell densities and increased levels of soluble and insoluble impurities. Our fiber chromatographic clarification solutions provide the ideal balance of innovative materials and efficient product recovery to streamline your filtration processes.

3M™ Harvest RC Chromatographic Clarifier platform

Streamline your processes by combining steps into a single solution with the 3M Harvest RC chromatographic clarifier platform. This single platform offers you two innovative solutions: 3M™ Harvest RC Chromatographic Clarifier for direct clarification and 3M™ Harvest RC Centrate Chromatographic Clarifier for centrate clarification. Both feature fiber chromatography technology and can enable you to replace multiple depth filtration steps while increasing your yield of therapeutic product.

The 3M™ Harvest RC Chromatographic Clarifier platform provides:

Consistent and comparable clarified fluid quality with either product
High capacity, synthetic, single-use products
Linear scalability from lab to manufacturing
Enhanced product recovery of >95%
Superior contaminant removal to <500 ppb DNA

See chromatographic clarifier details

De 3M™ Harvest RC familie met de 3M™ Harvest RC Chromatographic Clarifier BT500. 3M™ Harvest RC is de volgende generatie in oogst- en klaringstechnologie voor culturen met een hoge celdichtheid. Deze oplossing is ontworpen als een efficiënte optie om de opbrengsten van monoklonale antilichamen (mAb) te verhogen, terwijl het upstream proces wordt vereenvoudigd door de centrifuge- en/of dieptefiltratie-eenheidsoperaties te vervangen.

3M™ Emphaze™ AEX Hybrid Purifier

For removal of small soluble and insoluble contaminants alike, there’s the Emphaze AEX hybrid purifier. This solution dovetails with your current process to improve downstream purification, removing small contaminants and increasing yield.

Use it for secondary purification after your depth filtration step in processes not using a centrifuge, or in high turbidity applications where a centrifuge is used. The Emphaze AEX hybrid purifier can also stand alone for primary filtration in low turbidity applications not requiring a centrifuge.

In a representative bacterial lysate process, a decrease of soluble contaminants and increase of process robustness may be achieved by using Emphaze AEX hybrid purifier. Reduced impurities, including endotoxins, DNA and HCP, may lead to chromatography column protection (e.g., IMAC, CEX).

See hybrid purifier details

High performance depth filtration

A widely used approach to biopharmaceutical purification, depth filtration goes deep. It offers reliable filtration throughout the filtration media, particularly when there is a high particle load, compared to just the filter surface with traditional membrane filtration. Our family of 3M™ Zeta Plus™ Depth Filters are easy to handle and come in a variety of nominal pore sizes, formats, media types and configurations.

The single-use 3M™ Zeta Plus™ Encapsulated System offers scalability while delivering excellent performance in throughput and filtration efficiency with proper media selection and sizing.

Discover depth filtration

Bioprocessing technician loading capsules into holder

Selecting the right solution for your unique processes

Fiber chromatographic clarification solutions			Depth filtration
Solutions	3M™ Harvest RC Chromatographic Clarifier platform	3M™ Emphaze™ AEX Hybrid Purifier	3M™ Zeta Plus™ Depth Filters
Technology platform	Quaternary amine (Q) functionalized polypropylene nonwoven	Quaternary amine (Q) functionalized polypropylene nonwoven	Cellulose-based filtration media
Mechanism	AEX chromatography	AEX chromatography	Size-based exclusion Low to moderate charge-based exclusion
Materials	Synthetic	Synthetic	Cellulose, binder resin, filter aid
Feed stream	Harvest RC Chromatographic Clarifier: Direct harvest and clarification Harvest RC Centrate Chromatographic Clarifier: Centrate clarification	Clarification	Direct harvest and clarification (2 stages) Centrate clarification
Scalability	Linear scalability from lab to multi-capsule commercial production (including screening tools)	Linear scalability from lab to multi-capsule commercial production	Scalability from lab scale to multi-capsule production scale
Water flushable	Yes	No	Yes
Learn more	Discover fiber chromatography solutions	See product details: 3M™ Emphaze AEX Hybrid Purifier	Explore depth filtration solutions

School of Purification: Start learning today

Developed by our global experts, passionate about innovative approaches to biopharmaceutical separation and purification, School of Purification provides resources to help you and your team ramp up quickly. These free webinars and on-demand learning resources cover overcoming bottlenecks, optimizing efficiency, current trends, and future possibilities.

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